|Roberts, Phillip - University Of California|
Submitted to: Genbank
Publication Type: Other
Publication Acceptance Date: 8/27/2014
Publication Date: 9/21/2015
Citation: Ulloa, M., Roberts, P.A. 2015. DNA sequences and composition from 12 BAC clones-derived MUSB SSR markers mapped to cotton (Gossypium Hirsutum L. x G. Barbadense L.)chromosomes 11 and 21. Genbank. GeneBank accessions: KM396694-KM396705.
Technical Abstract: To discover resistance (R) and/or pathogen-induced (PR) genes involved in disease response, 12 bacterial artificial chromosome (BAC) clones from cv. Acala Maxxa (G. hirsutum) were sequenced at the Clemson University, Genomics Institute, Clemson, SC. These BACs derived MUSB single sequence repeat (SSR) markers, and SSRs were previously placed on the cotton chromosomes 11 and 21. These chromosomes are of interest because of the discovery of R or PR genes underlying QTLs involved in plant resistance-response to root-knot nematode (Meloidogyne incognita), reniform nematode (Rotylenchulus reniformis), Fusarium wilt (Fusarium oxysporum f.sp. vasinfectum), Verticillium wilt (Verticillium dahliae), and black root rot (Thielaviopsis basicola) on several of their genomic regions. The BAC clones were sequenced to an approximate 8X coverage, which resulted in three to eight ordered contigs spanning approximately 140,000 bp per contig. The clone sequence-composition of BAC-derived MUSB0404, MUSB0641, MUSB0827, MUSB0953, MUSB1000, MUSB1015, MUSB1035, MUSB1076, MUSB1163 and MUSB1278 SSRs from chromosome 11, and MUB0810 and MUSB0823 SSRs from chromosome 21 revealed sequence homology of receptor kinase proteins, early-responsive to dehydration stress proteins, universal stress proteins, auxin-responsive proteins, disease resistance and stress response proteins, and transposable elements (TE). TE included retrotransposon ty1-copia subclass, retrotransposon ty3-gypsy subclass, gag-pol polyprotein, mutant gag-pol polyprotein, mutator sub-class protein, and copia-like retrotransposable. The clone sequence information and physical mapping of BACs provides an additional genomic resource of these resistance gene-rich regions of the cotton genome on chromosomes 11 and 21. These BAC clone sequences will be deposited in GenBank (NCBI: http://www.ncbi.nlm.nih.gov). Continuing genetic and physical framework alignment of sequence information in cotton will help to expedite the discovery of R and PR genes and the assembly of a whole genome, eventually leading to breeding for disease resistance.