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ARS Home » Pacific West Area » Parlier, California » San Joaquin Valley Agricultural Sciences Center » Crop Diseases, Pests and Genetics Research » Research » Publications at this Location » Publication #304418

Title: Glassy-winged sharpshooter Microbiota explored using deep 16S rRNA sequencing from individual insects

item Rogers, Elizabeth
item Backus, Elaine

Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 5/15/2014
Publication Date: 6/22/2014
Citation: Rogers, E.E., Backus, E.A. 2014. Glassy-winged sharpshooter Microbiota explored using deep 16S rRNA sequencing from individual insects. In:Proceedings of the Hemipteran-Plant Interactions Symposium Abstract Book, p.76.

Interpretive Summary:

Technical Abstract: The glassy-winged sharpshooter (GWSS) is an invasive insect species that transmits Xylella fastidiosa, the bacterium causing Pierce’s disease of grapevine and other leaf scorch diseases. X. fastidiosa has been shown to colonize the anterior foregut (cibarium and precibarium) of sharpshooters, where it may interact with other bacterial species present. To evaluate such interactions, a survey of microbiota associated with the GWSS anterior foregut was conducted. A portion of the 16S rRNA gene was amplified and sequenced from 96 individual GWSS, 24 from each of 4 locations (field-collected in Bakersfield, CA, Ojai, CA, or Quincy, FL; and a laboratory colony originally collected in Bakersfield in 2003-2005). An average of approximately 150,000 sequence reads were obtained per insect. The most common genus detected was Wolbachia. X. fastidiosa was detected in all 96 individuals examined. By multilocus sequence typing, both X. fastidiosa subspecies fastidiosa and subspecies sandyi were present in GWSS from California and the colony; only subspecies fastidiosa was detected in GWSS from Florida. In addition to Wolbachia and X. fastidiosa, 23 other bacterial genera were detected at or above an average incidence of 0.1%; these included plant-associated microbes (Methylobacterium, Sphingomonas, Agrobacterium, and Ralstonia) and soil- or water-associated microbes (Anoxybacillus, Novosphingobium, Caulobacter, and Luteimonas). Sequences belonging to species of the family Enterobacteriaceae also were detected but it was not possible to assign these to individual genera. Chloroplast 16S rRNA also was amplified from dissected sharpshooter cibaria and precibaria.