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ARS Home » Plains Area » Lubbock, Texas » Cropping Systems Research Laboratory » Livestock Issues Research » Research » Publications at this Location » Publication #303906

Title: Supplementation of OmniGen-AF during the receiving period modulates the metabolic response to a lipopolysaccharide challenge in feedlot steers

item Sanchez, Nicole
item BUNTYN, JOE - University Of Nebraska
item Carroll, Jeffery - Jeff Carroll
item WISTUBA, TROY - Prince Agri Products, Inc
item DEHAAN, KEVIN - Prince Agri Products, Inc
item SIEREN, SARA - University Of Nebraska
item JONES, STEVEN - University Of Nebraska
item SCHMIDT, TY - University Of Nebraska

Submitted to: Journal of Animal Science Supplement
Publication Type: Abstract Only
Publication Acceptance Date: 4/10/2014
Publication Date: 7/25/2014
Citation: Sanchez, N.C., Buntyn, J.O., Carroll, J.A., Wistuba, T., Dehaan, K., Sieren, S.E., Jones, S.J., Schmidt, T.B. 2014. Supplementation of OmniGen-AF during the receiving period modulates the metabolic response to a lipopolysaccharide challenge in feedlot steers. Journal of Animal Science Supplement. 92(E-Supplement 2):250. Abstract #503.

Interpretive Summary:

Technical Abstract: The use of probiotic feed supplements to enhance animal health and growth are of great interest to the beef industry. Studies have demonstrated that some probiotic supplements may affect metabolism, and therefore influence an animal’s response to an immune challenge. This study was designed to determine the effect of supplementing feedlot steers with OmniGen-AF during the receiving period on the metabolic response to a lipopolysaccharide (LPS) challenge. Steers (n = 18; 270 ± 5 kilograms body weight) were obtained and transported to the University of Nebraska Agricultural Research and Development Center feedlot. Upon arrival steers were processed and separated into 2 treatment groups (n = 9/treatment): one group was fed a standard receiving diet (Control; Cont) and the other group was fed the same receiving diet supplemented with OmniGen-AF at 4 grams/45.4 kilograms body weight/day for 29 days (OmniGen-AF). On day 27 steers were fitted with indwelling jugular cannulas and placed in individual stalls. On day 28, steers were challenged intravenously with LPS (0.5 micrograms/kilograms body weight at 0 hour) and blood samples were collected at 30-minute intervals from -2 to 8 hours and at 24 hours post-challenge. Serum was isolated and stored at -80C until analyzed for glucose, non-esterified fatty acids (NEFA) and blood urea nitrogen (BUN) concentrations. Glucose concentrations were affected by treatment (P = 0.009) and time (P < 0.001). Glucose was greater in OmniGen-AF steers compared to Cont steers (76.4 ± 1.1 milograms/deciliter vs. 72.4 ± 1.0 milograms/deciliter). For NEFA concentrations, there was a treatment (P < 0.001) and time (P < 0.001) effect. Specifically, Cont (0.210 ± 0.007 milimoles/liter) steers had greater NEFA concentrations than OmniGen-AF steers (0.101 ± 0.010 milimoles/liter). There was a tendency (P = 0.07) for a treatment x time interaction such that NEFA concentrations were greater (P = 0.03) in Cont steers than OmniGen-AF steers from 3 to 8 h after LPS challenge. For BUN, there was a treatment (P < 0.001) effect such that concentrations were greater in Cont steers (12.4 ± 0.1 miligrams/deciliter) than OmniGen-AF supplemented steers (11.5 ± 0.1 milograms/deciliter) throughout the study, and were not affected by time (P = 0.28). These data suggest that OmniGen-AF supplementation modulates the metabolic response to a LPS challenge and provides an indication that supplementation of feedlot steers with OmniGen-AF may prevent the breakdown of other substrates (e.g., protein and fat) for energy during an immune challenge.