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ARS Home » Northeast Area » Beltsville, Maryland (BARC) » Beltsville Agricultural Research Center » Animal Biosciences & Biotechnology Laboratory » Research » Publications at this Location » Publication #303652

Research Project: Functional Genomics Approaches for Controlling Diseases of Poultry

Location: Animal Biosciences & Biotechnology Laboratory

Title: Evaluation of Montanide TM ISA 71 VG adjuvant during profilin vaccination against experimental coccidiosis

Author
item JANG, SEUNG - US Department Of Agriculture (USDA)
item KIM, DUK KYUNG - US Department Of Agriculture (USDA)
item Lillehoj, Hyun
item Lee, Sung
item LEE, KYUNG WOO - US Department Of Agriculture (USDA)
item BERTRAND, FRANCOIS - Seppic, Inc
item DUPUIS, LAURENT - Seppic, Inc
item DEVILLE, SEBASTIAN - Seppic, Inc
item BENAROUS, JULIETTE - Seppic, Inc
item LILLEHOJ, ERIK - University Of Maryland

Submitted to: American Association of Avian Pathologists
Publication Type: Abstract Only
Publication Acceptance Date: 4/13/2014
Publication Date: N/A
Citation: N/A

Interpretive Summary:

Technical Abstract: Chickens were immunized subcutaneously with an Eimeria recombinant profilin protein plus MontanideTM ISA 70 VG (ISA 70) or MontanideTM ISA 71 VG (ISA 71) water-in-oil adjuvants, or with profilin alone, and comparative RNA microarray analyses were performed to ascertain global transcriptomic changes induced by profilin/ISA 70 vs. profilin alone and by profilin/ISA 71 vs. profilin alone. Immunization with profilin/ISA 70 vs. profilin alone altered a greater number of transcripts compared with profilin/ISA 71 vs. profilin alone (509 vs. 296). Furthermore, alterations in transcript expression was evident for 10 canonical pathways in the profilin/ISA71 vs. profilin alone analysis compared to only two pathways for the profilin/ISA 70 vs. profilin alone analysis. Based on this analyses, ISA 71 was selected as a candidate adjuvant in conjunction with profilin vaccination for in vivo disease protection studies. Vaccination with profilin/ISA 71 was associated with greater body weight gain following E. acervulina infection, and decreased parasite fecal shedding after E. maxima infection, compared with profilin alone. Anti-profilin antibody levels were higher in sera of E. maxima- and E. tenella-infected chickens vaccinated with profilin/ISA 71 compared with profilin alone. Finally, the levels of transcripts encoding interferon-c, interleukin (IL)-2, IL-10, and IL-17A were increased in intestinal lymphocytes from E. acervulina-, E. maxima-, and/or E. tenella-infected chickens vaccinated with profilin/ISA 71 compared with profilin alone. None of these effects were seen in chickens injected with ISA 71 alone indicating that the adjuvant was not conferring non-specific immune stimulation. These results suggest that profilin plus ISA 71 augments protective immunity against specific Eimeria species in broiler chickens.