Submitted to: CDFA Pierce's Disease Control Program Research Symposium
Publication Type: Abstract Only
Publication Acceptance Date: 11/20/2013
Publication Date: 12/6/2013
Citation: Backus, E.A., Rogers, E.E. 2013. Optimizing EPG settings to record blue-green sharpshooter X waves for future studies of grape host plant resistance to Xf inoculation. CDFA Pierce's Disease Control Program Research Symposium. p. 3-10.
Technical Abstract: The long-term goal of the research reported in this review is to develop methodology for assessment of grapevine resistant to sharpshooter inoculation of Xylella fastidiosa(Xf)into healthy grapevines, thereby preventing Xf infection. Such a trait would be quite different from the more common mechanism of resistance to Xf, i.e., resistance to systemic bacterial spread and multiplication, after vector inoculation has already occurred. Identification and selection of an anti-inoculation trait depends upon two research objectives: 1) understanding behavioral mechanisms of Xf inoculation by sharpshooters, and 2) devising a rapid method for identifying grapevines that deter inoculation behaviors. Electrical penetration graph (EPG) monitoring of insect feeding research has revealed the behavioral mechanism of Xf inoculation. The inoculation behavior is represented by the sharpshooter EPG X wave. The present research showed that the same X wave components are visible for the blue-green sharpshooter as for the glassy-winged sharpshooter. Thus, the present work supports that: 1) EPG will be a useful technology for detection of Xf inoculation behaviors, 2) EPG monitor settings can be optimized for recordings of the blue-green sharpshooter X wave, and 3)those settings may be different from the best settings for glassy-winged sharpshooter recordings.