|Harrison, Robert - Bob|
|Jarvis, Donald - University Of Wyoming|
Submitted to: Book Chapter
Publication Type: Book / Chapter
Publication Acceptance Date: 12/14/2012
Publication Date: 1/29/2016
Citation: Harrison, R.L., Jarvis, D.L. 2016. Transforming Lepidopteran Insect Cells for Improved Protein Processing and Expression. Book Chapter. 359-379.
Technical Abstract: The lepidopteran insect cells used with the baculovirus expression vector system (BEVS) are capable of synthesizing and accurately processing foreign proteins. However, proteins expressed in baculovirus-infected cells often fail to be completely processed, or are not processed in a manner that meets a researcher’s needs. This chapter discusses a metabolic engineering approach that addresses this problem. Basically, this approach involves the addition of new or enhancement of existing protein processing functions in established lepidopteran insect cell lines. In addition to improvements in protein processing, this approach has also been used to boost the level of protein expression when using the BEVS. Methods for engineering cell lines and assessing their properties as improved hosts for the BEVS are detailed. Examples of lepidopteran insect cell lines engineered for improved protein N-glycosylation, folding/trafficking, and expression are described.