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ARS Home » Pacific West Area » Corvallis, Oregon » Forage Seed and Cereal Research » Research » Publications at this Location » Publication #260894

Title: High-throughput genotyping of hop (Humulus lupulus L.) utilising diversity arrays technology (DArT)

Author
item HOWARD, E - University Of Tasmania
item WHITTOCK, S - University Of Tasmania
item JAKSE, J - University Of Ljubljana
item CARLING, J - Diversity Arrays Technology
item MATTHEWS, P - Ss Steiner, Inc
item PROBASCO, G - John I Haas, Inc
item Henning, John
item DARBY, P - Wye Hops Ltd
item CERENAK, A - Slovenian Institute Of Hop Research And Brewing
item JAVORNIK, B - University Of Ljubljana
item KILIAN, A - Diversity Arrays Technology
item KOUTOULIS, A - University Of Tasmania

Submitted to: Theoretical and Applied Genetics
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 12/23/2010
Publication Date: 1/18/2011
Citation: Howard, E., Whittock, S.P., Jakse, J., Carling, J., Matthews, P.D., Probasco, G., Henning, J.A., Darby, P., Cerenak, A., Javornik, B., Kilian, A., Koutoulis, A. 2011. High-throughput genotyping of hop (Humulus lupulus L.) utilising diversity arrays technology (DArT). Theoretical and Applied Genetics. 122(7):1265-1280.

Interpretive Summary: Implementation of molecular methods in hop breeding is dependent on the availability of sizeable numbers of polymorphic markers and a comprehensive understanding of genetic variation. Diversity Arrays Technology (DArT) is a high-throughput cost-effective method for the discovery of large numbers of quality polymorphic markers without reliance on DNA sequence information. This study is the first to utilize DArT marker technology for identifying different hop varieties when confronted with a wide range of varieties from around the world. The markers produced from this time and cost efficient genotyping tool will be a valuable resource for numerous applications in hop breeding and genetics studies, such as mapping, marker assisted selection, genetic identity testing, guidance in the maintenance of genetic diversity and the directed breeding of superior cultivars.

Technical Abstract: Implementation of molecular methods in hop breeding is dependent on the availability of sizeable numbers of polymorphic markers and a comprehensive understanding of genetic variation. Diversity Arrays Technology (DArT) is a high-throughput cost-effective method for the discovery of large numbers of quality polymorphic markers without reliance on DNA sequence information. This study is the first to utilize DArT marker technology for hop (Humulus lupulus L.) genotyping, identifying 730 polymorphic markers from 92 hop accessions. The marker quality was similar to DArT markers previously generated for other species. Genetic relationships in hop illustrated by DArT coincide with knowledge generated using alternate marker systems. The markers produced from this time and cost efficient genotyping tool will be a valuable resource for numerous applications in hop breeding and genetics studies, such as mapping, marker assisted selection, genetic identity testing, guidance in the maintenance of genetic diversity and the directed breeding of superior cultivars.