|FORGE, T - Agriculture Canada|
Submitted to: Society of Nematologists
Publication Type: Abstract Only
Publication Acceptance Date: 4/30/2010
Publication Date: 9/1/2010
Citation: Zasada, I.A., Forge, T.A. 2010. Identification and istribution of Pratylenchus spp. on blueberry. Society of Nematologists. 42(3):278.
Technical Abstract: Pratylenchus spp. are commonly encountered in blueberry (Vaccinium spp.) fields in the Pacific Northwest of the United States and coastal British Columbia of Canada. However, in controlled experiments blueberry does not appear to be a host for P. penetrans. We sampled commercial blueberry fields in the spring and fall to determine the species of Pratylenchus associated with blueberry plantings and to determine the geographical distribution of these species. Pratylenchus spp. were more commonly found in soil samples (68% and 61% in spring and fall, respectively) than in blueberry root samples (42% and 50% in spring and fall, respectively). Population densities averaged 160 and 201 Pratylenchus spp./100 g soil and 82 and 69 Pratylenchus spp./g dry root in the spring and fall, respectively. Across locations, Pratylenchus spp. were detected in 56% and 54% of the sampled weed roots in spring and fall, respectively, at population densities averaging 1,063 and 1,354 Pratylenchus spp./g dry root in spring and fall, respectively. The species of Pratylenchus present in blueberry fields was determined by amplification of the D2/D3 expansion domains of the nuclear 28S rDNA subunit.