|Bonman, John - Mike|
Submitted to: Journal of Plant Registrations
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 7/30/2010
Publication Date: 1/1/2011
Citation: Verhoeven, E.C., Bonman, J.M., Bregitzer, P.P., Brunick, B., Cooper, B., Corey, A.E., Cuesta-Marcos, A., Filichkina, T., Mundt, C.C., Obert, D.E., Rossnagel, B., Richardson, K.L., Hayes, P.M. 2011. Registration of the BISON genetic stocks in Hordeum vulgare L. Journal of Plant Registrations. 5:135-140. Interpretive Summary: Fighting barley stripe rust epidemics can be done effectively by growing barley cultivars containing genes that make the plants partially or completely resistant to the stripe rust fungus. Often these genes are in "wild" barley lines that are not fit for commercial production, so breeding must be done to transfer these genes into experimental breeding lines and, eventually, to improved cultivars. This research report details production of experimental lines by the transfer of multiple resistance genes into the popular feed barley, Baronesse. Nine separate lines were produced, each containing either a separate resistance gene, or combinations of different resistance genes. These stripe rust resistant lines will be useful as parents for breeding new stripe rust resistant lines. In addition, these lines will help scientists study and understand the interactions of these genes with the barley stripe rust fungus. Each of these experimental lines, plus the "wild" barley parents donating the resistance genes, have been entered into the USDA-ARS National Small Grains Collection for preservation and distribution to interested scientists and breeders.
Technical Abstract: A set of near-isogenic lines (NILs) was developed in barley (Hordeum vulgare ssp. vulgare) at Oregon State University, and evaluated for resistance to barley stripe rust (BSR, incited by Puccinia striiformis f.sp. hordei) and agronomic potential. The NILs, henceforth the BISON lines (Barley stripe rust resistance ISOgeNic) represent BSR resistance QTL alleles introgressed individually and in all possible combinations into a susceptible background ‘Baronesse’. The lines and donors with experimental line names and registration are: BISON 1H (Reg. No. ;PI ), BISON 4H (Reg. No. ;PI ), BISON 5H (Reg. No. ;PI ), BISON 7H (Reg. No. ;PI ), BISON 1H +4H (Reg. No. ;PI ), BISON 1H+5H (Reg. No. ;PI ), BISON 4H+5H (Reg. No. ;PI ), BISON 1H+4H+5H (Reg. No. ;PI ), BISON 0-QTL (Reg. No. ;PI ), BCD 12 (Reg. No. ;PI ), BCD47 (Reg. No ;PI ), and D3-6/B23 (Reg. No. ;PI ). All BISON lines, resistance allele donors, and susceptible background parent ‘Baronesse’ were evaluated in five environments and phenotypic data, in conjunction with genotypic data were used to characterize QTL allele introgressions, and to asses the impact of these introgressions and pyramiding on agronomic performance. Across all environments, no significant difference in grain yield was observed. The triple allele introgression line (BISON 1H+4H+5H) was significantly shorter and had a lower percentage of plump kernels in all environments. All lines, excluding one, showed significantly decreased rates of BSR severity relative to the control and susceptible parent. Together with genotypic data, these lines provide a valuable resource for evaluating the effectiveness of specific QTL, QTL pyramiding, and quantitative versus qualitative disease resistance. Barley breeding programs may also use this germplasm for introgessing resistance alleles.