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ARS Home » Pacific West Area » Albany, California » Western Regional Research Center » Crop Improvement and Genetics Research » Research » Publications at this Location » Publication #252164

Title: Application of intragenic technology for development of disease-resistant potato

item Ponciano, Grisel
item ROMMENS, CAIUS - Jr Simplot Company
item Rockhold, David
item McCue, Kent
item Whalen, Maureen
item Belknap, William

Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 3/25/2010
Publication Date: 8/7/2010
Citation: Ponciano, G.P., Rommens, C.M., Rockhold, D.R., Mc Cue, K.F., Whalen, M.C., Belknap, W.R. 2010. Application of intragenic technology for development of disease-resistant potato. The American Phytopathological Society Annual Meeting, August 7-11, 2010, Nashville, TN.

Interpretive Summary:

Technical Abstract: Intragenics (also known as ‘cisgenesis’) is a plant transformation technology that consists of employing only genes, regulatory, and transfer DNA sequences from the plant genus to be transformed. Current status of our use of the technology to produce disease-resistant potatoes will be presented. The late blight disease caused by Phytophthora infestans continues to be potato’s most serious disease worldwide. Using an intragenic transformation vector developed by J.R. Simplot Company, and the recently cloned resistance (R) gene from Solanum bulbocastanum, RB (also known as Rpi-blb1), effective against a wide spectrum of P. infestans races, we aim to develop a late blight-resistant potato. We are also developing a strategy for intragenic potato plants resistant to viruses. We took a computational approach to identify virus homologous sequences present in the potato genome. The identified genomic sequences are being evaluated to determine their potential to serve as small interfering RNA (siRNA) templates for suppression of viral gene expression, and/or micro RNA (miRNA) templates to induce plant viral disease resistance via RNA interference (RNAi).