Submitted to: Plant and Animal Genome Conference Proceedings
Publication Type: Proceedings
Publication Acceptance Date: 12/3/2009
Publication Date: 1/8/2010
Citation: Oliver, R.E., Kilian, A., Ladizinsky, G., Korol, A., Lazo, G.R., Jellen, R., Lutz, J., Tinker, N., Anderson, J.M., Dumlupinar, Z., Wisniewski, N.H., Campbell, R.D., Shackelford, I., Jackson, E.W. 2010. Evolution of the Oat Genetic Road Map: From Tetraploid to Hexaploid. Proc. Plant and Animal Genome XVIII. Pg. 142. Conference Proceedings. Interpretive Summary:
Technical Abstract: The development of a genetic linkage map for hexaploid oat (Avena sativa L. 2n = 6 x = 42) that defines all 21 chromosomes has been hindered due to the lack of oat-based markers and the size and complexity of the oat genome. Recent efforts in oat DArT, SSR, and SNP marker development should improve map resolution. In addition, development of a complete tetraploid linkage map with DArT, SSR, and SNP markers complementary to a hexaploid map would allow direct alignment and possibly the elucidation of marker positions in the more complex hexaploid genome. The objectives of this work were to: i) expand the existing DArT marker array to include representations from A. maroccana (2n = 4x =28), ii) develop new oat-based SSR and SNP markers, iii) construct a tetraploid linkage map with markers complementary to the Ogle1040/TAM O-301 linkage map, and iv) align the maps. Avena maroccana lines from seven Moroccan locations, along with the Ba13-13/A169 (BA) parental lines, were used to develop tetraploid-based DArT clones. The new DArT representations were then used along with the existing hexaploid DArT array to genotype the 11 lines and 113 BA recombinant inbred lines. New hexaploid-derived SSR and SNP markers were also added to the BA population. We will present diversity statistics and new molecular linkage maps. In addition, we will present alignments between the tetraploid and hexaploid maps and discuss the potential evolutionary relationships.