Location: Crop Germplasm ResearchTitle: Plastid markers in Carya Author
|Grauke, Larry - L J|
Submitted to: Pecan National Workshop Proceedings
Publication Type: Proceedings
Publication Acceptance Date: 8/31/2009
Publication Date: N/A
Citation: N/A Interpretive Summary:
Technical Abstract: We evaluated 8 "universal" plastid primers and qualified 3 as polymorphic and informative within Carya. Pecans of known lineage and geographic origin, representatives of other Carya species and interspecific hybrids were profiled. In an analysis of 169 Carya accessions, 21 alleles were observed among the three loci, and combined to form 16 unique plastid microsatellite (cpSSR) haplotypes. Open pollinated seedlings of a common maternal parent were indistinguishable from each other and the seed parent. Sympatric species of the genus share common plastid haplotypes, while Asian species and the Mexican endemic Carya palmeri have unique profiles. Of the 151 pecans profiled, the greatest diversity was observed in Mexican pecan populations with 9 haplotypes, 5 of which were found only in Mexico. Northern US pecan populations were characterized by two haplotypes also found in species of Section Carya and in Mexico. PCR primers capable of tracing maternal inheritance in Carya have broad applications for characterizing founder populations of individual stands and for conservation and improvement of pecan.