|O'LEARY, CF - University Of Richmond|
|TREONIS, A - University Of Richmond|
|MARKS, C - University Of Richmond|
Submitted to: Soil Ecology Meeting Abstracts
Publication Type: Abstract Only
Publication Acceptance Date: 4/15/2009
Publication Date: N/A
Technical Abstract: We developed a taxa-specific, fluorescence in situ hybridization (FISH) technique to localize microbial nucleic acids within nematode bodies. This technique involves hybridization of a nucleic acid probe to target microbial sequences. Hybridization is detected microscopically, as the probes have fluorescent labels attached to them. Here, we present an overview of this molecular microscopy technique and a photo essay portraying several applications. Using bacteria-specific rRNA FISH probes, this method has been used to successfully localize the passage of microbial food sources through the esophageal region of Caenorhabditis elegans. The same technique was applied to bacterial-feeding nematodes (Cephalobidae spp.) isolated from soils from Death Valley, CA. We were also able to detect the presence of archaeal rRNA within these nematodes, using archaea-specific FISH probes. To our knowledge, this is the first time that consumption of archaea by nematodes has been documented. We have also been able to visualize intestinal symbionts using this technique. Phylogenetic staining is a promising tool for the study of nematode food sources and symbionts.