Submitted to: Journal of Chromatography A
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 12/30/2008
Publication Date: 2/17/2009
Citation: Koerner, J.L., Lee, J., Kennedy, J.A. 2009. Determination of proanthocyanidin A2 content in phenolic polymer isolates by reversed-phase high performance liquid chromatography. Journal of Chromatography A. 1216:1403-1409. Interpretive Summary: This paper describes method optimization and identification of proanthocyanidin A2 isolated from cranberries and peanuts.
Technical Abstract: This article summarizes the development of an analytical method for the determination of proanthocyanidin (PAC) A2 in phenolic polymer isolates following acid-catalyzed degradation in the presence of excess phloroglucinol. Isolates from cranberry juice concentrate (CJC) were extensively characterized and molar extinction coefficients were determined for the terminal A2 and phloroglucinol adduct of the extension A2 unit. Peanuts are also reported to contain both extension and terminal A-type PACs; therefore, a total peanut system (TPS) was chosen to test the effectiveness of the HPLC method that was developed with the CJC system. Kinetic studies were conducted and reaction conditions were optimized for the A2 units in both CJC and TPS. The optimized method provides quantitative and reproducible information on the A2 content of proanthocyanidin isolates.