Submitted to: Molecular Breeding
Publication Type: Peer reviewed journal
Publication Acceptance Date: 12/17/2008
Publication Date: 1/4/2009
Citation: Kelley, K., Whitworth, J.L., Novy, R.G. 2009. Mapping of the potato leafroll virus resistance gene, Rlretb, from Solanum etuberosum identifies interchromosomal translocations among its E-genome chromosomes 4 and 9 relative to the A-genome of Solanum L. sect. Petota. 2009 Molecular Breeding 23:489-500. Interpretive Summary: Potato leafroll virus (PLRV) causes yield and quality reductions in potato. Currently, none of the primary potato varieties grown in the United States have host plant resistance to this virus. A gene for resistance to PLRV, Rlretb has previously been identified and characterized in our research program. The chromosome location of Rlretb,was determined in order to facilitate the use of the gene for developing PLRV-resistant potato varieties.
Technical Abstract: Gene Rlretb, derived from the potato species Solanum etuberosum, confers resistance to potato leafroll virus (PLRV). Mapping of this gene would aid in developing marker-assisted selection protocols to facilitate its introgression into cultivated potato. One RFLP marker and 45 cleaved amplified polymorphic markers (CAPs) markers were used to screen an etuberosum-derived BC3 family segregating for PLRV resistance conferred by Rlretb. Nine markers from linkage group 4 of the tomato map displayed linkage with Rlretb, however eight additional markers from linkage group 4 that should have been syntenic with Rlretb were not. Instead they segregated with 12 markers previously mapped to linkage group 9 of the tomato map, indicative that chromosomes 4 and 9 of S. etuberosum have translocated regions relative to the potato and tomato genomes. These chromosomal translocations have placed Rlretb beyond the end of the published map of linkage group 4 of tomato with the closest marker, C2_At1g42990, mapping 13.6 cM from Rlretb.