Submitted to: Biotechnology for Fuels and Chemicals Symposium Proceedings
Publication Type: Abstract Only
Publication Acceptance Date: 3/6/2008
Publication Date: 5/5/2008
Citation: Lee, C.C., Wagschal, K.C., Accinelli, R., Orts, W.J., Robertson, G.H., Wong, D. 2008. An alpha-glucuronidase enzyme activity assay adaptable for solid phase screening. Biotechnology for Fuels and Chemicals Symposium Proceedings. Abstract 6-26.
Technical Abstract: Glucuronic acid is a common chemical moiety that decorates the xylan polymer of hemicellulose. This chemical substituent impairs both anzymatic and acidic hydrolysis of xylosidic bonds. The alpha-glucuronidase enzyme hydrolyzes the 1,2-linked glucuronic acid from the terminal, non-reducing xylose of xylo-oligosaccharides. There are relatively few fÑ-glucuronidase genes in the public databases. We have developed an assay with commercially-available reagents that can be used to search DNA libraries for fÑ-glucuronidase genes in a high throughput, solid phase activity screen.