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ARS Home » Plains Area » Lubbock, Texas » Cropping Systems Research Laboratory » Plant Stress and Germplasm Development Research » Research » Publications at this Location » Publication #225992
Title: Transcriptomics and proteomics of drought tolerance in peanuts

Author
item KOTTAPALLI, KAMESWARA RAO - TTU
item PUPPALA, NAVEEN - NEW MEXICO STATE UNIVERSI
item BUROW, MARK - TEXAS A&M UNIVERSITY
item Payton, Paxton

Submitted to: Experimental Biology
Publication Type: Abstract Only
Publication Acceptance Date: 4/5/2008
Publication Date: 4/9/2008
Citation: Kottapalli, K., Puppala, N., Burow, M., Payton, P.R. 2008. Transcriptomics and proteomics of drought tolerance in peanuts. Experimental Biology 2008. San Diego, California. April 5-9, 2008.

Interpretive Summary:

Technical Abstract: Two peanut mini-core accessions exhibiting divergent responses to water-deficit stress were identified from a suite of physiological screening assays. In the present study we employed a combined transcriptomics and proteomics approach to study both the primary transcriptional networks and functional proteins involved in the drought tolerance mechanism by comparing the stress tolerant genotype (COC041) to a stress susceptible genotype (COC166). High-density oligonucleotide arrays (Agilent Technologie 8 x 15k), containing all peanut ESTs in the public domain were used for transcript profiling. One- and two-dimensional gel electrophoresis was performed on leaf soluble protein extracts from drought-stressed and control tissues. This combined approach revealed that lipoxygenase, an enzyme of jasmonic acid biosynthesis induced under drought may be involved in drought signaling. Similarly, induction of the fatty acid biosynthesis enzyme, acetyl-CoA carboxylase carboxyl transferase, in the tolerant accession suggests a possible role of altered lipid biosynthesis in the drought tolerance phenotype. Suppression of lectin proteins and induction of aldolases were also measured and are likely consequences of a general abiotic stress response in the tolerant peanut accession. These data represent the first step in identifying key regulatory genes and pathways responsible for divergent stress response phenotypes in peanut and the identification of potential candidates for marker-assisted breeding.