|Mendoza Herrera, Maria|
|Grauke, Larry - L J|
Submitted to: Plant and Animal Genome Conference
Publication Type: Abstract Only
Publication Acceptance Date: 10/5/2007
Publication Date: 12/1/2007
Citation: Mendoza Herrera, M.A., Grauke, L.J., Binzel, M.L. 2007. Confirmation of SSR markers within a structured collection of pecan [Carya illinoinesis (Wangenh.) K. Koch], cultivars [abstract]. Plant and Animal Genome Conference XVI, January 12-16, 2008, San Diego, California. Paper No. P140. Interpretive Summary:
Technical Abstract: Simple Sequence Repeat (SSR) markers have proven to be a powerful tool for characterizing genetic diversity, investigating population structure, verifying cultivar identity and parentage, and aiding selection in breeding programs. The USDA ARS National Clonal Germplasm Repository for Pecans and Hickories (NCGR-Carya) has developed and refined a group of SSR markers to evaluate accessions of Carya with the goal of developing marker aided selection for use in pecan breeding. A structured collection of specific controlled cross progeny and their parents was developed from verified Repository accessions in order to confirm the utility of additional SSR markers. Eight SSRs derived from a microsatellite-enriched library have been previously qualified as informative and formed the basis of comparison in the current work. Eight prospective SSRs derived from microsatellite loci of Juglans nigra and eight developed from pecan EST sequences were tested. Five new SSR markers were qualified as informative for pecan: 4 from the Juglans primers and 1 developed from an EST sequence. Markers were selected based on amplification and polymorphism within the cultivars analyzed. Reproducibility and consistency were tested by using different sources of DNA for the same cultivar. DNA was extracted from leaf samples collected from the same tree in different years or from different trees of the cultivar. At least two samples of each cultivar were scored to give the final result. Utility of the SSR markers in cultivar identification was demonstrated with the confirmation of controlled crosses released by the breeding program.