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ARS Home » Pacific West Area » Aberdeen, Idaho » Small Grains and Potato Germplasm Research » Research » Publications at this Location » Publication #219998

Title: DArT marker development and applications in oat

item Jackson, Eric
item Rines, Howard
item Anderson, Joseph

Submitted to: Plant Genome Conference Proceedings
Publication Type: Abstract Only
Publication Acceptance Date: 12/1/2007
Publication Date: 1/12/2008
Citation: Tinker, N.A., A. Kilian, E. Jackson, and the OAT DArT Consortium. DArT marker development and applications in oat.. Plant and Animal Genome XVI. San Diego, CA. 2008

Interpretive Summary:

Technical Abstract: Progress of genomic research in oat has been limited by a lack of common markers and consensus maps that would provide integration platforms for structural genomic analysis. Diversity Array Technology (DArT) is a strategy that provides a high density of molecular markers that can be tested in parallel for a reasonable cost. In addition to providing tools for large-scale collaborations, such as association analysis, these markers will enable smaller and more focused studies to be integrated into a complete knowledge base of oat genomics. An international consortium of researchers from 13 institutions* has collaborated with DArT P/L in Australia to develop the groundwork for DArT markers in oat. We have identified and cloned approximately 2000 selectively-amplified DNA fragments that show polymorphism in a panel of 200 cultivated oat varieties. Of these, approximately 1000 have been mapped in the Kanota x Ogle RI population. An additional 2000 clones showed polymorphism in a sample of 16 accessions from related oat species. We will present an analysis of genetic diversity based on DArT markers, and an updated genetic map in the Kanota x Ogle. We will discuss how this work can enhance parallel efforts in oat SSR development, and we will propose new strategies by which the oat research community can develop and utilize improved, physically-anchored consensus maps.