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ARS Home » Pacific West Area » Albany, California » Western Regional Research Center » Bioproducts Research » Research » Publications at this Location » Publication #218536

Title: Cloning and characterization of xylanase gene from hot spring microorganism

item Lee, Charles
item Kibblewhite, Rena
item Smith, Michael
item Wagschal, Kurt
item Orts, William
item Wong, Dominic

Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 9/13/2007
Publication Date: 10/11/2007
Citation: Lee, C.C., Accinelli, R., Smith, M.R., Wagschal, K.C., Orts, W.J., Wong, D. Cloning and characterization of xylanase gene from hot spring microorganism. Meeting Abstract #307.

Interpretive Summary:

Technical Abstract: Lignocellulosic biomass has the potential to supply many of our fuel and chemical feedstocks that are currently derived from fossil fuel sources. Xylan is one of the largest fractions of lignocellulose and must be hydrolyzed to simpler components to harness its full potential. We cultured microorganisms from samples collected from a hot springs environment that produced enzymes to break down xylan. We cloned a glycosyl hydrolase family 11 xylanase gene from a hot springs bacterium. The recombinant protein was expressed in Escherichia coli, purified, and biochemically characterized.