Submitted to: International Congress of Plant Pathology Abstracts and Proceedings
Publication Type: Abstract Only
Publication Acceptance Date: 12/4/2007
Publication Date: 8/24/2008
Citation: Bonman, J.M., Gu, Y., Coleman Derr, D., Jackson, E.W., Chao, S., Bockelman, H.E. 2008. Origin of barley accessions with multiple disease resistance determined by SSR analysis.. International Congress of Plant Pathology Abstracts and Proceedings.
Technical Abstract: Although only 1% of accessions of cultivated barley (Hordeum vulgare subsp. vulgare L.) in the USDA National Small Grains Collection (NSGC) are of unknown origin, these accessions represent 20% of the accessions with multiple disease resistance (MR). These accessions were originally obtained in 1930 from an N.I. Vavilov nursery in Kharkov, Ukraine. Since many MR accessions in the NSGC originated from Ethiopia, the purpose of this study was to test the hypothesis that the unknown MR accessions were also from Ethiopia. The experiment used 40 simple sequence repeat (SSR) markers to screen 12 MR and 12 susceptible (S) accessions of unknown origin, 12 MR and 12 S accessions from Ethiopia, and 152 accessions randomly chosen from the NSGC barley core subset. SSRs that failed to amplify products in >10% of the genotypes and individual alleles occurring in < 5% of the accessions were omitted from the analysis. Results from 24 SSRs amplifying 148 alleles were used to group accessions based on using Ward’s clustering procedure. Ten of the MR accessions of unknown origin were clearly grouped with accessions from Ethiopia. The grouping included 10 MR, 5 S, and 3 core subset accessions from Ethiopia and one S accession of unknown origin. Our results support the idea that the MR accessions collected from the Vavilov nursery were originally from Ethiopia. This initial experiment is being repeated using 48 SSRs, 192 accessions, and a more automated genotyping procedure.