Submitted to: Plant and Animal Genome
Publication Type: Abstract Only
Publication Acceptance Date: 12/16/2005
Publication Date: 1/9/2006
Citation: Jessup, R.W., Burson, B.L., Broussard, A.N., Krishnaramanujam, R., Engelke, M.C., Genovesi, A.D., Reinert, J.A., Kamps, T.L., Schulze, S., Paterson, A.H. 2006. An EST-SSR and AFLP-based genome map of zoysiagrass [abstract]. Plant and Animal Genome XIV Conference. Paper No. P370.
Technical Abstract: Zoysiagrass (Zoysia matrella L.) is a highly drought- and salt-tolerant grass that is grown extensively across the central and southern United States as a lawn and turf grass. It has also been utilized as a forage grass. Zoysiagrass is a tetraploid (2n=4x=40), has a protogynous flowering behavior, and is highly cross-pollinated. In recent years, breeders have developed improved turf cultivars, but additional breeding efforts are needed to reduce injury from biotic and abiotic stresses. Therefore, the objective of this research was to construct a detailed linkage map of zoysiagrass to serve as a framework for marker-assisted breeding of the species. Three hundred and fifty AFLPs and 330 EST-SSRs were mapped across 90 F1 hybrids, yielding linkage maps for the maternal and paternal parents. Linkage data was used to estimate genome size, chromosome pairing, type of polyploidy, and recombination levels. Fall armyworm resistance was scored across the mapping population and included in the linkage analyses. Implications of the genome map for marker-assisted breeding and zoysiagrass improvement programs will be discussed.