Submitted to: American Journal of Potato Research
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 8/8/2004
Publication Date: 1/1/2005
Citation: Gillen, A.M. and Novy, R. 2005. Development of SSRs and Conversion of RFLP Markers to PCR-Based Markers for Introgression of Viral Resistance Genes from Solanum etuberosum. American Journal of Potato Research. 82 (1):70.
Technical Abstract: Potato virus X (PVX), potato virus Y (PVY) and potato leafroll virus (PLRV) are important viral pathogens of potato. Solanum etuberosum, a wild relative of potato, is a source of resistance to these viruses that has yet to be fully exploited by plant breeders. A 1 EBN species, S. etuberosum cannot be readily hybridized with diploid or tetraploid clones of the cultivated potato and its E-genome is divergent from the A-genome of cultivated potato. Somatic hybridization between S. etuberosum (2n=2x=24) and a Gp. Tuberosum haploid x S. berthaultii hybrid (2n= 2x=24) was employed to overcome barriers to sexual hybridization. Somatic hybrids have been successfully crossed to cultivated potato (2n=4x=48) and resistances to PVX, PVY and PLRV have been found to segregate in the BC2 progeny. We have correlated RFLP markers that are unique to the S. etuberosum parent with the viral resistance phenotypes of a BC2 population. Genes for resistance to PVY and PLRV have been localized to four different regions of the E-genome. Further marker saturation of these regions, along with the rest of the E-genome, is required to further define the regions of interest for the development of molecular markers tightly-linked to viral resistance genes. Use of these markers for marker-aided selection will greatly facilitate breeding efforts. In addition, this data will be used to evaluate the impact of homology on intergenomic recombination and the possible preferential transmission of certain S. etuberosum chromosomes. We will report on progress to develop SSR markers and the conversion of RFLP markers to PCR-based markers. Further marker analysis in the BC3 will be done to identify markers closely linked to resistance genes.