Submitted to: Keystone Symposia
Publication Type: Abstract Only
Publication Acceptance Date: 2/19/2004
Publication Date: 2/19/2004
Citation: Burke, J.J., Chen, J., Xin, Z., Velten, J.P. 2004. Molecular mapping of arabidopsis genes contributing to thermotolerance [abstract]. Keystone Symposia.
Technical Abstract: Acquired thermotolerance deficient mutants have been isolated based upon their heat sensitivity in a "chlorophyll Accumulation Bioassay" reported previously by our laboratory (Plant Physio. 123:575-588. 2000). Map-based cloning of the AtTS02 mutant identified a mutation in digalactosyldiacylglycerol synthase (DGDG1, At3g11670). A second mutation site in the DGDG1 gene was identified in AtTS100 and AtTS104, which belong to the same complementation group as AtTS02. Phenotypic analysis of the Salk_113941 T-DNA insertion into DGDG1 also showed heat sensitivity in the Chlorophyll Accumulation Bioassay. Mapping studies of AtTS223, and EMS mutant in the Columbia ecotype, show that the mutation is localized to Chromosome 1. Another Columbia mutant, AtTS244, has been mapped to a region on Chromosome 5. A thermotolerant-deficient line obtained from the WS2 ecotype also maps to a region on Chromosome 5 that is genetically distinct from the AtTS244 mutation. We screened a collection of Salk T-DNA insertion lines and have identified 21 lines exhibiting thermotolerant-deficient phenotypes. These Salk lines include sites on all five chromosomes, and physiologically are associated with membrane biosynthesis and stability, in addition to specific heat shock proteins. Special thanks to Joseph R. Ecker and the ARBC for providing the T-DNA Salk lines described above.