Submitted to: Plant and Animal Genome
Publication Type: Abstract Only
Publication Acceptance Date: 12/16/1996
Publication Date: N/A
Citation: Reddy, A., Haisler, R.M., Yu, J., Kohel, R.J. 1997. AFLP mapping in cotton [abstract]. Plant and Animal Genome V Conference. Paper No. P80.
Technical Abstract: Amplified Fragment Length PolymorphismTM has proven to be a rapid and reliable source of genetic markers. We have used this method to develop an AFLP linkage map of cotton genome using an F2 population from an interspecific cross between Gossypium hirsutum acc. TM-1 and G. barbadense acc. 3-79. The parents were selected for their productivity and fiber quality respectively. We have adapted several of the available methods for ALFP marker analysis both automated and manual including the ALFP Plant Mapping Kit (Perkin Elmer ABI) which uses fluorescently labeled primers and fully automated analysis system when coupled with Genotyper 1.1 software, and the AFLP Analysis System 1 (Life Technologies) which provides protocols for both 33P and 32P labeled primers and relies on autoradiography to analyze the markers with manual scoring. For developing a cotton AFLP linkage map we have used a combination of both fluorescently and 33P labeled primers to generate AFLP markers. Using the 64 available AFLP primer combinations 490 markers were generated, scored, and analyzed with Mapmaker 3.0 operating on a UNIX system. An AFLP linkage map generated from this data will be presented.