|ALBA, ROB - BOYCE THOMPSON INSTITUTE
|MOORE, SHANNA - CORNELL UNIVERSITY
Submitted to: Plant Biotechnology Journal
Publication Type: Book / Chapter
Publication Acceptance Date: 9/1/2002
Publication Date: 7/2/2003
Citation: PAYTON, P.R., ALBA, R., MOORE, S.L. GENE EXPRESSION PROFILING. PLANT BIOTECHNOLOGY. 2003. HANDBOOK OF PLANT BIOTECHNOLOGY; CHAPTER 2. 2003. P. 1-9.
Technical Abstract: DNA microarray technology is fast becoming a standard tool for gene expression analysis. The laboratory methods and protocols for array construction, processing, and hybridization are well established. Many of the initial plant genome sequencing projects are providing large sets of expressed sequence tag (EST) or full length complimentary DNA (cDNA) clones and printed arrays to the public for use in gene expression studies. With the completion of sequencing the Arabidopsis genome, biologists are studying gene expression patterns on a global scale. Also taking advantage of the rapid availability of sequence data, commercial entities are now providing oligonucleotides arrays or sets for arraying. Although transcript monitoring is currently the most popular use for arrays, they have been successfully utilized in fields ranging from mutation detection to evolutionary sequence analysis. Microarray publications seem to appear with increasing fervor, however very few concern work in the plant sector. Here we provide a basic outline of gene expression profiling in plants using DNA microarrays, the advantages to ESTs and oligonucleotides, probe synthesis, and data analysis.