Submitted to: American Society of Plant Biologists Annual Meeting
Publication Type: Abstract only
Publication Acceptance Date: 8/3/2002
Publication Date: 8/7/2002
Citation: Tripathy, D., Klueva, N., Wang, J., Mellacheruvu, M., Payton, P.R., Allen, R. 2002. Transcription profiling of drought stress-induced expressed sequence tags during cotton boll formation[abstract]. American Society of Plant Biologists, August 3-7, 2002, Denver, Colorado. CDROM. Interpretive Summary: Not required
Technical Abstract: Cotton is one of the world's major crops and is grown in more than 80 countries worldwide. One of the major factors limiting fiber yield and lint quality in cotton is drought stress following bloom. Many genes are expressed specifically during fiber initiation, development, and maturation. As drought has the greatest effect during boll formation, it is important to understand the genes specifically regulated or expressed during drought stress. A cDNA library was constructed from drought stressed 8-10 dpa cotton bolls. The 5' ends of 1032 randomly selected clones were sequenced in order to identify potentially informative genes. The Blast X algorithm was used to translate each EST nucleotide sequence into six possible reading frames and compared with a protein sequence database available through National Center for Biotechnology Information (NCBI). Sequence homology search indicated that 496 (48.1%) EST's exhibited significant similarities to characterized database entries and 41 of them represented previously identified cotton genes and proteins; 536 (51.9%) had no significant similarities to sequence with known functions. These ESTs were divided into 12 categories depending upon gene functions. cDNA microarray analysis was done with the EST clones to find genes involved in fiber development and stress responses during critical stages of boll formation.