|Klein, Robert - Bob|
Submitted to: Genetics
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 2/18/2002
Publication Date: 5/1/2002
Citation: Islam-Faridi, M.N., Childs, K.L., Klein, P.E., Hodnett, G., Menz, M.A., Klein, R.R., Rooney, W.L., Mullet, J.E., Stelly, D.M., Price, H.J. 2002. A molecular cytogenetics map of sorghum chromosome 1: Fluorescence in situ hybridization analysis with mapped bacterial artificial chromosomes. Genetics. 161:345-353. Interpretive Summary: A major advancement in biology hinges on a detailed understanding of the cell including the architecture of chromosomes. Chromosomes harbor the genes in all living organisms and while all chromosomes have a similar architecture, the structure of each chromosome is unique. Detailed pictures of chromosomes called karyotypes can reveal a blueprint of chromosomes. With a karyotype of a chromosome, landmarks can be located along with the regions of the chromosome that contain genes and these landmarks can make improving plants more efficient. This study details our cost-effective methods to make a very descriptive blueprint of the architecture of the first of 10 chromosomes of sorghum. As sorghum is a close relative to rice, corn and wheat, this chromosomal blueprint will be valuable to scientists working on important grass crops. Information will be primarily used by fellow scientists.
Technical Abstract: We used structural genomic resources for sorghum to target and develop multiple molecular cytogenetic probes that would provide extensive coverage for a specific chromosome of sorghum. Bacterial artificial chromosome clones containing molecular markers mapped across sorghum linkage group A were labeled as probes for fluorescence in situ hybridization. Signals from single, dual and multi probes of bacterial artificial chromosomes to spreads of chromosomes were associated with the largest sorghum chromosome. The order of individual bacterial artificial chromosomes loci along the chromosome was fully concordant to that of marker loci along the linkage map. The centromere and nucleolus organizing region were located in a large heterochromatic region that spans approximately sixty percent of chromosome 1. Variation in recombination frequency among euchromotic chromosomal regions also was apparent. The successful development of multi probe cocktails for fluorescence in situ hybridization suggests that it is feasible to develop chromosome specific paints from genomic resources that provide an especially powerful framework for mapping.