|Klein, Robert - Bob|
Submitted to: Genome
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 11/2/2001
Publication Date: 4/1/2002
Citation: Kim, J., Childs, K.L., Faridi, N., Menz, M.A., Klein, R.R., Klein, P.E., Price, J.H., Mullet, J.E., Stelly, D.M. 2002. Integrated karyotyping of sorghum by in situ hybridization of landed BACs. Genome. 45:402-412. Interpretive Summary: A major advancement in biology hinges on a detailed understanding of the cell including the architecture of chromosomes. Chromosomes harbor the genes in all living organisms and while all chromosomes have a similar architecture, the structure of each chromosome is unique. Detailed pictures of chromosomes, called karyotypes, can reveal a blueprint of chromosomes. With a karyotype of a chromosome, landmarks can be located along with the regions of the chromosome that contain genes and these landmarks can make improving plants more efficient. This study details our cost-effective methods to make a detailed blueprint of the architecture of each of the 10 chromosomes of sorghum. As sorghum is a close relative to rice, corn, and wheat, these chromosomal blueprints will be valuable to scientists working on important grass crops. Information will be primarily used by fellow scientists.
Technical Abstract: The reliability of genome analysis and proficiency of genetic manipulation are increased by assignment of linkage groups to specific chromosomes, placement of centromeres, and orientation with respect to telomeres. We have endeavored to establish means to enable these steps in sorghum, the genome of which contains 10 chromosomes. Our approach relies on fluorescence in situ hybridization and integrated structural genomic resources, including large insert genomic clones in bacterial artificial chromosomes libraries. To develop robust fluorescence in situ hybridization probes, we selected 22 bacterial artificial chromosomes that encompass the 10 linkage groups. The results indicate that linkage and physical maps of sorghum allow facile selection of bacterial artificial clones according to position and fluorescence in situ hybridization quality. This capability will enable development of a high-quality molecular cytogenetic map and an integrated genomics system for sorghum.