Submitted to: Acta Horticulturae
Publication Type: Proceedings
Publication Acceptance Date: 6/10/2001
Publication Date: 11/1/2002
Citation: PUSEY, P.L. INTERRELATIONSHIP OF TEMPERATURE, FLOWER DEVELOPMENT, AND BIOLOGICAL CONTROL OF FIRE BLIGHT. ACTA HORTICULTURAE. 2002. v. 590. p. 247-251. Interpretive Summary: Fire blight is a serious disease of apple and pear trees. Antibiotics applied to blossoms have become less effective because of resistance in the causal organism, and biological control has been considered as a viable alternative. The commercial product BlightBan (J.R. Simplot Co., Boise, ID) with the active ingredient, Pseudomonas fluorescens strain A506, became available to U.S. growers in 1996. However, experience with this and other beneficial organisms for control of fire blight indicates an inconsistency possibly related to environment and the plant host. Advances in biological control will partly depend on an understanding of these factors. Therefore, a study was done to examine the interrelationships of temperature, pollination, stigma age, and microbial colonization and interactions. It was noted that strain A506 failed to multiply at late blossom stages that did allow growth of the disease agent and other beneficial bacteria. This and other results will be of value in selecting biological control agents and optimizing their use for fire blight.
Technical Abstract: BlightBan (a.i., Pseudomonas fluorescens strain A506) has been available in recent years and other biological agents (e.g., Pantoea agglomerans strains C9-1 and E325) are being developed for fire blight control. Advances will partly depend on an understanding of interrelationships involving environment, flower development and senescence, and microorganisms. Laboratory experiments were performed with detached blossoms of crab apple (Malus sp. 'Manchurian'). When inoculated stigmas were held at various temperatures, strain A506 failed to grow at the upper temperature range of Erwinia amylovora, but strains C9-1 and E325 had ranges extending beyond that of the pathogen. When flowers were subject to various temperatures and incubation periods before inoculation, the duration of stigma conduciveness to bacterial multiplication decreased as temperature increased, and it was shorter when flowers were pollinated versus non-pollinated. These interactions were confirmed with mature apple trees (Malus x domestica Borkh.'Gala') surrounded by plastic enclosures with heating and cooling to maintain different temperature ranges. In trials in 2000 and 2001, temperatures during bloom averaged 13.4 and 14.1 C at the low range and 21.5 and 21.9 at the high range, respectively. The longest period stigmas retained the capacity to support bacterial growth when inoculated was about 14 days, occurring with non-pollinated flowers at low temperatures. The shortest period was 3 or 4 days, occurring with pollinated flowers at high temperatures. Results were similar with different bacteria, however strain A506 failed to multiply at late stages of stigma senescence, which did allow growth of the pathogen and strain E325. On crab apple flowers, antagonist strains differed in their capacity to reduce pathogen populations and these differences were consistent during the period when stigmas were conducive to pathogen growth.