Submitted to: Federation of American Societies for Experimental Biology Conference
Publication Type: Abstract Only
Publication Acceptance Date: 1/5/2002
Publication Date: 3/1/2002
Citation: LI, K., PAYTON, P.R., GU, W., MCCLUNG, J.P., LEI, X. MICROARRAY ANALYSIS OF BONE METABOLISM GENE EXPRESSION IN CELLULAR GLUTATHIONE PEROXIDASE (GPX1) KNOCK-OUT MICE. FEDERATION OF AMERICAN SOCIETIES FOR EXPERIMENTAL BIOLOGY CONFERENCE. 2002. SUPPLEMENT V. 16. NO. 4-5. Interpretive Summary: SUMMARY NOT REQUIRED
Technical Abstract: The objective of this study was to analyze the regulation of genes implicated in bone metabolism and disease by cellular glutathione peroxidase (GPX1) using microarray analysis. RNA was extracted and purified from 3 wild-type (WT) and 3 GPX1 knockout (KO) mice (10-wk old). cDNA chips were then utilized to detect the differential expression of genes between KO and WT mice. Labeling and detection methods were conducted according to the Expression Array Detection Kit (Genisphere Inc., Montvale, NJ) protocol with minor modifications. Chips were analyzed using the Gene Pix 4000 Array Scanner (Axon Instruments, Foster City, CA) and Gene Pix Pro Software. 41 of the 150 genes on the chip demonstrated changes. Among these 41 genes, 5 are down-regulated and 36 are up-regulated in KO mice. This study demonstrates the usefulness of microarray technology in identifying GPX1-sensitive gene targets for future study of bone metabolism and disease.