Submitted to: American Society of Plant Biologists Annual Meeting
Publication Type: Abstract Only
Publication Acceptance Date: 4/25/2002
Publication Date: 8/8/2002
Citation: MCCARTOR, K., PAYTON, P.R., PATHAN, M.S., ROSENOW, D.T., NGUYEN, H.T. TRANSCRIPTIONAL PROFILING OF DROUGHT RESPONSES IN SORGHUM. AMERICAN SOCIETY OF PLANT BIOLOGISTS ANNUAL MEETING. 2002. SUPPLEMENT 76 P. 48 Interpretive Summary: NO SUMMARY REQUIRED
Technical Abstract: The identification of genetic factors underlying the complex responses of plants to drought stress provides a solid basis for improving drought resistance. The stay-green character in sorghum (Sorghum bicolor L. Moench) is a post-flowering drought resistance trait, which makes plants resistant to premature senescence under drought stress during the grain filling stage. Previous mapping experiments identified four quantitative trait loci (QTLs) for the stay-green trait. These QTLs contain genes for photosynthetic carbon metabolism, heat shock proteins, and abscisic acid responsive genes. The objective of this study is to integrate phenotypic analysis, physiological data, and expression profile data to further characterize genes controlling the stay-green trait. Recombinant-inbred (RILs) and near-isogenic lines (NILs) obtained from a cross between B35 (drought resistant/stay-green parent) and Tx7000 (susceptible parent) were scored for the stay-green trait following drought treatment. We have identified 2 RILs and 2 NILs, which have been scored for either presence or absence of the drought-resistant phenotype, that will be used in the expression profiling experiments. Two drought-induced, subtracted cDNA libraries were constructed using the B35 and Tx7000 parental lines. 10,000 cDNAs were sequenced and annotated in the laboratory of L. Pratt at the University of Georgia-Athens. A 9,000 element cDNA microarray is being constructed for gene expression analysis of these lines. A detailed description of the stay-green trait, phenotypic characterization, and expression profiling data will be presented at this meeting.