Location: Horticultural Crops Production and Genetic Improvement Research Unit
Project Number: 2072-21000-060-004-I
Project Type: Interagency Reimbursable Agreement
Start Date: Jul 30, 2021
End Date: Jul 29, 2023
Objective:
Objective 1: Develop a multiplex real-time RT-PCR for screening detection of tomato torrado virus (ToTV) in plants and seeds to include internal plant control.
Objective 2: Develop a conventional RT-PCR for confirmatory detection of ToTV in plants and seeds matrices to be combined with sequencing for identification.
Objective 3: Develop a multiplex real-time RT-PCR for detection of several torradoviruses infecting tomato.
Approach:
Primers and probes will be developed to known sequences of ToTV and existing tomato infecting torradoviruses. For the ToTV multiplex real-time RT-PCR, two primer/probe sets to target y capsid (coat) protein (CP) and RNA-dependent RNA Polymerase (RdRp) will be selected and evaluated. For the ToTV conventional RT-PCR, primer sets will be developed to the most characteristic gene sequences of size that provide enough sensitivity and specificity.
