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ARS Home » Midwest Area » St. Paul, Minnesota » Cereal Disease Lab » Research » Research Project #440795

Research Project: Development of Genomic Tool for Cereal Crop Improvement

Location: Cereal Disease Lab

Project Number: 5062-21220-025-016-S
Project Type: Non-Assistance Cooperative Agreement

Start Date: Aug 31, 2022
End Date: Aug 30, 2024

Objective:
Specific objectives of this project are: 1) Develop a multi-component viral replicon system with large DNA carrying capacity for genome editing in planta; 2) Optimize the system to achieve high efficiency genome modification through homology-directed repair; and 3) Design a simple and high-throughput screening method of infected plants to allow rapid identification of critical plants.

Approach:
The underlying mechanism for generating advantageous variations in the plant genome is through: 1) recombination, 2) mutagenesis, and/or 3) transformation/genome editing. We propose to overcome the limitations of these methods by developing a novel viral vector system to enable rapid and site-specific plant genome modification, adaptable to a multitude of genotypes and species, without tissue culture. Successful completion of this project will lead to a paradigm shift in crop improvement and detailed analysis of plant development. For the first time, this study is aimed at developing a tool that can be used in planta for the desired genetic modifications in multitude of plant species and genotypes.