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ARS Home » Midwest Area » St. Paul, Minnesota » Cereal Disease Lab » Research » Research Project #438409

Research Project: Genomics and Molecular Diagnostics of Puccinia graminis and Associated Rust Fungi

Location: Cereal Disease Lab

Project Number: 5062-21220-023-33-S
Project Type: Non-Assistance Cooperative Agreement

Start Date: Aug 1, 2020
End Date: Jul 31, 2021

Objective:
Develop genomic tools for Puccinia graminis and associated rust fungi infecting Berberis and Mahonia; Develop molecular tools for genotyping and detection.

Approach:
Selected isolates and samples of P. graminis species complex and rust fungi infecting the alternate hosts of P. graminis (Berberis and Mahonia) will be sequenced. Sequence data will be mined for allelic variation of selected genes, as well as, genome-wide single nucleotide polymorphisms (SNPs). Comparative genomics will be used to identify gene alleles and SNPs that will be used to develop diagnostic assays.