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ARS Home » Southeast Area » Griffin, Georgia » Plant Genetic Resources Conservation Unit » Research » Research Project #438240

Research Project: Screening USDA/ARS Capsicum Germplasm for the Presence of Tomato Brown Rugose Fruit Virus (ToBRFV)

Location: Plant Genetic Resources Conservation Unit

Project Number: 6046-21000-012-18-A
Project Type: Cooperative Agreement

Start Date: May 15, 2020
End Date: May 14, 2021

Objective:
The principle objectives of the project are: 1.) Evaluate (index) 1246 seed inventories in the USDA/ARS Capsicum germplasm collection for the presence of Tomato Brown Rugose Fruit Virus (ToBRFV), 2.) Sequence the PCR product(s) of samples that test positive and compare with known strains of ToBRFV. 3.) Isolate infected seed inventories and restrict distribution. 3.) Update the GRIN-Global database to reflect the test results for all indexed seed inventories.

Approach:
Approximately 1246 seed inventories (200 seed each) were drawn from the Capsicum germplasm collection (Griffin, GA), packaged, and sent to AgDia Testing Services. Nucleic acids were extracted from all samples. All samples will be indexed for the presence of ToBRFV via indexing for Tobamoviruses. PCR products of all samples testing positive will be sequenced to identify ToBRFV-infected samples. All test results and sequence data will be returned to the Griffin site and used to update the GRIN-Global database and modify seed distribution practices as warranted.