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ARS Home » Southeast Area » Griffin, Georgia » Plant Genetic Resources Conservation Unit » Research » Research Project #438232

Research Project: Screening the USDA/ARS Collection of Capsicum Germplasm for the Presence of Tomato Brown Rugose Fruit Virus (ToBRFV)

Location: Plant Genetic Resources Conservation Unit

Project Number: 6046-21000-012-17-A
Project Type: Cooperative Agreement

Start Date: May 15, 2020
End Date: May 14, 2021

Objective:
The principle objectives of the project are: 1.) Evaluate (index) seed inventories in the USDA/ARS Capsicum germplasm collection for the presence of Tomato Brown Rugose Fruit Virus (ToBRFV)/Tobamo Group). 2.) Sequence the PCR product(s) of samples that test positive and compare with known strains of ToBRFV. 3.) Update the GRIN-Global database to reflect the test results for all indexed seed inventories.

Approach:
Approximately 3000 seed inventories (100 to 200 seed each) will be drawn from the Capsicum germplasm collection (Griffin, GA), packaged, and sent to AgDia Testing Services. All samples will be indexed for the presence of Tobamovirus Group members followed by the sequencing of the PCR products of all samples testing positive, and the comparison of the sequence data with known strains of ToBRFV. All test results and sequence data will be returned to the Griffin site and used to update the GRIN-Global database.