Location: Plant Germplasm Introduction and Testing Research
Project Number: 2090-21000-037-005-S
Project Type: Non-Assistance Cooperative Agreement
Start Date: Aug 20, 2018
End Date: Aug 19, 2023
Objective:
Objective is to increase seed protein concentration in yellow pea cultivars by:
1. Determine the genetic variation of protein and mineral nutrient concentration in current cultivars and advanced breeding lines of yellow pea (G x E).
2. Identify single nucleotide polymorphisms (SNPs) associated with alleles controlling seed protein concentration in pea using Genome Wide Association Study (GWAS).
3. Develop and validate breeder friendly markers for increasing seed protein and mineral nutrient concentration in new yellow pea cultivars.
Approach:
Objective 1. Determine the genetic and environmental variation of protein and mineral nutrient concentration in current cultivars and advanced breeding lines of yellow pea.
Field Experiment: A field study of 30 current and advanced breeding lines of yellow pea cultivars will be planted using a Randomized Complete Block (RCB) design with four replications each year for four years, in three environments [Montana (2), Washington]. New lines will be entered in years 3 and 4 (see Breeding section below). Soil tests will be conducted each year and standard practices for herbicide and pesticide applications will be followed. Agronomic data will include plant density, plant height, days to flowering and maturity, seed size and seed yield. Harvested seed will be analyzed for protein concentration, total starch, crude fat, moisture, ash, and total fiber using near infrared (NIR) spectrometry (NIR Matrix-I Spectrometer)]. Mineral nutrient concentrations will be determined using inductively coupled plasma mass spectrometry (ICP-MS) at cooperator core facilities. Expected results: We expect to understand the stability of current and future yellow pea cultivars for seed protein and mineral nutrients and prepare extension bulletins for growers and processors.
Objective 2. Identify SNPs associated with alleles controlling protein concentration in pea using a genome-wide association study (GWAS).
Field Experiment: A field study of 500 yellow pea lines including diverse plant genetic resources from the USDA world collection and cultivars, advanced breeding lines from the G x E Field Study (Obj. 1) will be planted using a RCB design with four replications over three years. Soil tests will be conducted each year and standard practices for herbicide and pesticide applications will be followed. Harvested seed will be analyzed for protein concentrations [Near Infrared (NIR) spectrometry (NIR Matrix-I Spectrometer)].
Genotyping: Genotyping-by-sequencing (GBS) will be conducted following the procedures of Elshire et al. (2011) successfully deployed for pea by Holdsworth et al. (2017).
Objective 3. Develop and validate breeder friendly markers for increasing protein and mineral nutrient concentration in new yellow pea cultivars. For significant SNPs identified in the seed protein concentration marker-trait associations (this proposal) and our published SNPs for mineral nutrient concentration (Ma et al. 2017a), two allele-specific forward primers and one common reverse primer will be designed (LGC, KBiosciences, Hoddesdon, UK)(Ma et al. 2017b).
