Location: Floral and Nursery Plants Research
Project Number: 8020-21000-072-00-D
Project Type: In-House Appropriated
Start Date: Apr 14, 2018
End Date: Apr 13, 2023
Objective 1: Establish long-term experimental hydrangea populations, and develop information management systems and standards for hydrangea phenotyping and genetic mapping to accelerate the development of disease and stress resistant hydrangea cultivars with superior product quality. [NP301, C1, PS1A; NP301, C4, PS4A] Sub-objective 1.A. Establish and phenotype Hydrangea macrophylla experimental populations for identification of disease resistance and ornamental traits. Sub-objective 1.B. Establish, genotype, and phenotype a range-wide Hydrangea quercifolia collection for natural genetic variation for disease resistance and ornamental traits. Sub-objective 1.C. Develop new phenotyping methods for important traits and create associated data management systems for genomic and phenotypic data. Objective 2: Develop, evaluate, and release improved germplasm of hydrangea and other high-value, native, and underrepresented nursery crop species to expand market opportunities for producers, stimulate consumer interest, and mitigate the environmental footprint of nursery production. [NP301, C1, PS1B] Sub-objective 2.A. Accelerate the development of superior Hydrangea germplasm using ploidy manipulations, and wide hybridization. Sub-objective 2.B. Evaluate germplasm including Hydrangea, Osmanthus, and Hamamelis for nursery production and breeding improvement potential.
During the next five years, Hydrangea and Osmanthus selections from previous breeding cycles will be progagated and distributed to cooperators. Germplasm evalutations of new species and cultivars will ensure a broad base for future cultivar development. We will confirm the three major powdery-mildew resistance SNPs in the two most resistant and two most susceptible cultivars using real-time PCR with TaqMan probes. Controlled crosses between the most powdery mildew-resistant and most susceptible cultivar are underway. This F1 mapping population will be genotyped via RT-PCR in the same manner as the mapping parents. This population will be planted in the field for a minimum three-year observation period. During this time, natural powdery mildew incidence and/or induced powdery mildew infection will be recorded. Digital images of each plant will be obtained in regular intervals throughout the growing season. Image analysis will be used to monitor the progression of disease infection and the number and size of new inflorescences. Together, the genotype and phenotype data will be used to refine the genome-wide association analysis, determine the efficacy of marker-assisted selection for powdery mildew resistance at the seedling stage in Hydrangea, and identify plants for further gene expression analysis. For genetic and horticultural analysis of oakleaf hydrangea, we will sample up to 20 populations within each state on a longitudinal/latitudinal cline, as well as representing the full diversity of environments in which the species occurs. Within each of these populations we will sample up to 20 plants. Three to five recently expanded leaves will be collected from selected plants, stored flat in Ziploc bags and stored in coolers with unique identifiers linked with each bag. Plant morphology, location, and other notes will be recorded using the EpiCollect5 web application. Leaves will be shipped overnight via UPS to the USDA-ARS in McMinnville, TN for processing, DNA isolation, and storage. Genotyping-by-sequencing will be performed by the University of Minnesota Genomics core facility. Bioinformatic support will be provided by the ARS NEA Statistics Group. Ten populations per state on a longitudinal/latitudinal cline, as well as representing the full diversity of environments in which the species occurs, will be evaluated for current generation estimates of genetic diversity and levels of inbreeding and for common garden horticultural characterizations in Minnesota, Mississippi, and Tennessee. Each location will evaluate 25-50 individuals of each population (60 populations total) for growth, form, floral, and disease resistance traits. Over 1500 F2 seedlings derived from hybridizations between 'Snow Queen' and the compact cultivars Pee Wee and Sikes Dwarf were evaluated for compact plant habit, quality and quantity of inflorescences, disease resistance and fall foliage color. Seven plants from this population have been selected for compact form and superior floral display. Seed and seedlings from these selected plants will be used for development of disease-screening methods.