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ARS Home » Southeast Area » Mississippi State, Mississippi » Crop Science Research Laboratory » Corn Host Plant Resistance Research » Research » Research Project #434375

Research Project: Enhanced Resistance of Maize to Aspergillus flavus Infection, Aflatoxin Accumulation, and Insect Damage

Location: Corn Host Plant Resistance Research

Project Number: 6064-21000-015-00-D
Project Type: In-House Appropriated

Start Date: Feb 8, 2018
End Date: Feb 7, 2023

Objective:
1. Identify new sources of maize germplasm with resistance to Aspergillus (A.) flavus infection and aflatoxin accumulation. 1.A: Screen maize germplasm obtained from the GEM project, CIMMYT, and other sources for resistance to aflatoxin accumulation to identify new and potentially useful sources of resistance. 1.B: Determine optimal combinations of A. flavus and A. parasiticus isolates for screening maize germplasm for resistance to aflatoxin accumulation under different environmental conditions. 2. Identify new sources of maize germplasm with resistance to fall armyworm, southwestern corn borer, and corn earworm. 2.A: Screen maize germplasm from CIMMYT, the GEM project, and other sources for resistance to leaf feeding by fall armyworm and southwestern corn borer. 2.B: Screen maize germplasm for resistance to ear feeding by fall armyworm, southwestern corn borer, and corn earworm. 3. Develop and characterize genetic mapping populations, identify and elucidate functions of genes associated with resistance, and develop molecular markers for enhancing maize germplasm resistance to A. flavus/aflatoxin and insects. 3.A: Identify genetic loci associated with resistance to A. flavus infection and aflatoxin accumulation via analysis of linkage and association mapping and sequencing data. 3.B: Identify genetic loci associated with resistance to lepidopteran insect feeding via analysis of linkage and association mapping, pathway analysis, or sequencing data. 3.C: Identify genes and elucidate functions of genes associated with resistance to A. flavus/aflatoxin and insects. 4. Develop and release maize germplasm with resistance to Aspergillus flavus infection, aflatoxin accumulation, and insect damage. 4.A: Develop and release maize germplasm lines with resistance to A. flavus infection and aflatoxin accumulation using conventional breeding methods. 4.B: Develop and release lines with resistance to feeding by southwestern corn borer and fall armyworm using conventional breeding methods. 4.C: Develop lines with resistance to A. flavus infection, aflatoxin accumulation, and insects using molecular markers and release together with marker information.

Approach:
Objective 1: Identify new sources of maize germplasm with resistance to Aspergillus (A.) flavus infection and aflatoxin accumulation. Screen maize germplasm obtained from the Germplasm Enhancement of Maize project (GEM) project, International Center for Maize and Wheat Improvement (CIMMYT), and other sources for resistance to aflatoxin accumulation to identify new and potentially useful sources of resistance. Determine optimal combinations of A. flavus and A. parasiticus isolates for screening maize germplasm for resistance to aflatoxin accumulation under different environmental conditions. Objective 2: Identify new sources of maize germplasm with resistance to fall armyworm, southwestern corn borer, and corn earworm. Screen maize germplasm from CIMMYT, the GEM project, and other sources for resistance to leaf feeding by fall armyworm and southwestern corn borer. Screen maize germplasm for resistance to ear feeding by fall armyworm, southwestern corn borer, and corn earworm. Objective 3: Develop and characterize genetic mapping populations, identify and elucidate functions of genes associated with resistance, and develop molecular markers for enhancing maize germplasm with resistance to flavus/aflatoxin and insects. Identify genetic loci associated with resistance to A. flavus infection and aflatoxin accumulation via analysis of linkage and association mapping and sequencing data. Identify genetic loci associated with resistance to lepidopteran insect feeding via analysis of linkage and association mapping, pathway analysis, or sequencing data. Identify genes and elucidate functions of genes associated with resistance to A. flavus/aflatoxin and insects. Objective 4: Develop and release maize germplasm with resistance to A. flavus infection, aflatoxin accumulation, and insect damage. Develop and release maize germplasm lines with resistance to A. flavus infection and aflatoxin accumulation using conventional breeding methods, and develop and release lines with resistance to feeding by southwestern corn borer and fall armyworm using conventional breeding methods. Develop lines with resistance to A. flavus infection, aflatoxin accumulation, and insects using molecular markers and release together with marker information.