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ARS Home » Pacific West Area » Albany, California » Western Regional Research Center » Foodborne Toxin Detection and Prevention Research » Research » Research Project #428134

Research Project: Development of a Hybridoma Data Base for Biological Toxins and Biothreat Agents

Location: Foodborne Toxin Detection and Prevention Research

Project Number: 2030-42000-049-04-I
Project Type: Interagency Reimbursable Agreement

Start Date: Mar 11, 2015
End Date: Sep 30, 2019

Objective:
Develop a data base of well characterized hybridoma cell lines (obtained from DHS cooperators) producing monoclonal antibodies to bacterial toxins and biothreat agents.

Approach:
Hybridoma cell lines will be obtained from DHS (estimated 20 to 50). Each hybridoma will be cultured, a number of frozen ampules prepared and stored in liquid nitrogen. Media from each line will be collected and the antibody produced characterized as to isotope. Additionally, when antigen is available, antibody binding to immobilized antigen evaluated. Frozen stocks will be maintained and samples made available to other U.S. Government agencies (e.g., FDA, CDC) as requested by DHS.