Methods & Techniques

   Double labeled water technique for daily energy expenditure.

         ••  ELISAand Multiplex Techniques to assess inflammatory markers, endocrine hormones and markers of bone metabolism.

         ••  ICP for mineral analysis for blood or urine. 

         ••  ⁴¹Ca tracer for labeling of bone mineral calcium and thus a direct measure of calcium resorption from the skeleton.  This technique is used in collaboration the Lawrence Livermore National Laboratory using accelerator mass spectrometry.