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United States Department of Agriculture

Agricultural Research Service

Research Project: GENETIC ENHANCEMENT FOR RESISTANCE TO BIOTIC AND ABIOTIC STRESSES IN HARD WINTER WHEAT Title: Rubisco activase and wheat productivity under heat stress conditions

Authors
item Ristic, Zoran
item Momcilovic, I - KANSAS STATE UNIV
item Bukovnik, Urska - KANSAS STATE UNIV
item Prasad, P.V. Vara - KANSAS STATE UNIV
item Fu, Jianming - KANSAS STATE UNIV
item DE Ridder, Benjamin - GRINNELL COLLEGE
item Elthon, E. Thomas - UNIV OF NEBRASKA-LINCOLN
item Mladenov, Novica - SERVIA-INST FIE&VEG CROPS

Submitted to: Journal of Experimental Botany
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: July 3, 2009
Publication Date: August 11, 2009
Repository URL: http://jxb.oxfordjournals.org/content/60/14/4003.short
Citation: Ristic, Z., Momcilovic, I., Bukovnik, U., Prasad, P., Fu, J., De Ridder, B., Elthon, E., Mladenov, N. 2009. Rubisco activase and wheat productivity under heat stress conditions. Journal of Experimental Botany. 60:4003-4014.

Interpretive Summary: Rubisco activase (RCA), an enzyme involved in photosynthesis, is subject to thermal inactivation in plants that are under heat stress. In this study, we investigated the possible relationship between relative amounts of RCA and plant yield in eleven European cultivars of winter wheat following prolonged exposure to heat stress. In addition, we also examined the effect of a short-term heat stress on RCA accumulation in four genotypes of wheat, five genotypes of maize and one genotype of Arabidopsis. Heat stress affected the accumulation of RCA in a few genotypes of wheat and maize but not in Arabidopsis. A significant positive linear correlation was found between the accumulation of wheat RCA and plant productivity under heat stress conditions. The results support the hypothesis that the endogenous level of RCA could play an important role in plant productivity under high temperature conditions.

Technical Abstract: Rubisco activase (RCA) constrains the photosynthetic potential of plants at high temperature (heat stress). We hypothesized that endogenous levels of RCA could serve as an important determinant of plant productivity under heat stress conditions. In this study, we investigated the possible relationship between expression levels of RCA and plant yield in eleven European cultivars of winter wheat following prolonged exposure to heat stress. In addition, we also examined the effect of a short-term heat stress on RCA expression in four genotypes of wheat, five genotypes of maize and one genotype of Arabidopsis thaliana. Immunoblots prepared from leaf protein extracts from control plants showed three RCA cross-reacting bands in wheat and two RCA cross-reacting bands in maize and Arabidopsis. The molecular mass of the observed bands was in the range between 40 and 46 kD. Heat stress affected the expression of RCA in a few genotypes of wheat and maize but not in Arabidopsis. In wheat, heat stress slightly modulated the relative amounts of RCA in some cultivars. In maize, heat stress did not seem to affect the existing RCA isoforms (40 and 43 kD) but induced accumulation of a new putative RCA of 45 – 46 kD. The new putative 45 – 46 kD RCA was not seen in a genotype of maize (ZPL 389) that has been shown to display an exceptional sensitivity to heat stress [Ristic and Cass (1993) J Plant Physiol 142: 759-764]. A significant positive linear correlation was found between the expression of wheat 45 – 46 kD RCA and plant productivity under heat stress conditions. The results support the hypothesis that endogenous levels of RCA could play an important role in plant productivity under supra-optimal temperature conditions.

Last Modified: 12/20/2014
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