|Behle, Robert - 3620-35-00, BRDC|
|Brandhorst, Tristan - J WHITTIER BIOL|
|Keanealy, William - J WHITTIER BIOL|
Submitted to: Aflatoxin Elimination Workshop Proceedings
Publication Type: Abstract Only
Publication Acceptance Date: October 24, 1995
Publication Date: N/A
Technical Abstract: Male nematodes were found in two species in addition to the fungal vector, the dusky sap beetle. A commercial strain of antifungal bacteria in flour formulation survived a year on the shelf and inhibited mold in axils and ears when applied as granules. A DNA-based method of identification of an insect fungal pathogen was developed for tracking dispersal from autoinoculators. A fermenting gel attractant for sap beetles was developed that was effective for weeks. Susceptibility to aflatoxin and A. flavus may be due to an absence and/or lack of inducibility of peroxidases. Leaf feeding on peroxidase over and underexpressing tobacco by caterpillars was dependent on leaf age and prior feeding. A fungal protein was toxic to three species of insects. A line of corn expressing B.t. crystal protein throughout the plant was tested for indirect resistance to fungi through inhibition of insect feeding. Corn flour encapsulated malathion often was more effective in controlling ear feeding insects with highly reduced levels of active ingredient and no harm to insect predators. It reduced mold infestation by three-fold in two varieties.