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United States Department of Agriculture

Agricultural Research Service

Research Project: ADVANCED CONVERSION TECHNOLOGIES FOR SUGARS AND BIOFUELS: SUPERIOR FEEDSTOCKS, PRETREATMENTS, INHIBITOR REMOVAL, AND ENZYMES

Location: Bioenergy Research Unit

Title: Purification and characterization of xylooligosaccharides (XOS) from Miscanthus x giganteus

Authors
item Chen, Ming-Hsu -
item Rajan, Kalavathy -
item Bowman, Michael
item Carrier, Danielle -
item Dien, Bruce
item Rausch, Kent -
item Tumbleson, M -
item Singh, Vijay -

Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: September 29, 2013
Publication Date: N/A

Technical Abstract: Our previous investigation showed that xylooligosaccharides (XOS) could be produced effectively from Miscanthus x giganteus (MxG). Using autohydrolysis, an XOS yield of to 13.5% (w/w) of initial biomass and xylan yield of 69.2% (w/w) was observed. In this study, we investigated the purification of XOS from hydrolyzed liquid. An activated carbon adsorption procedure was set up for recovering XOS and was followed by elution at five different ethanol solution. Most of the XOS eluted out with 30%, 50% ethanol/water solution; highest recovery of 47.9% (w/w) of the initial XOS could be achieved. XOS with lower degree of polymer (DP) resulted in better recovery. Fractions from activated carbon elution had purity up to 75% (w/w). By using centrifugal partition chromatogram (CPC), we can isolate xylobiose (X2) and xylotriose (X3) at the purity of 64% and 65%, respectively. Further ESI-MS spectrums showed the major molecular weight of X2 are 305.1 (X2-Na) and 321.1(X2-K); major molecular weight of X3 are 437.1 (X3-Na) and 453.1 (X3-K).

Last Modified: 4/21/2014
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