Location: Commodity Protection and Quality Research
Project Number: 2034-43000-043-081-R
Project Type: Reimbursable Cooperative Agreement
Start Date: Jul 1, 2025
End Date: Jun 30, 2026
Objective:
1. Expand and improve the gene editing approach in NOW through reliable gene insertion.
2. Develop a stable sterile NOW by mutating the male sterility factor.
Approach:
This research aims to improve the existing CRISPR protocol in order to establish a functional genetic control toolbox for navel orangeworm (NOW). Specific focus will be to enhance the efficiency of CRISPR mediated knock-out via optimizing the egg harvesting and injection survival. Further, a gene knock-in protocol specific to NOW will be developed. Target genes for eventual field deployment will be identified through investigating the reproductive physiology of this insect.