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ARS Home » Pacific West Area » Parlier, California » San Joaquin Valley Agricultural Sciences Center » Crop Diseases, Pests and Genetics Research » Research » Research Project #448073

Research Project: Screening of Locally Isolated Chitinase-Producing Bacteria for Biological Control of Powdery Mildew in Grapevine

Location: Crop Diseases, Pests and Genetics Research

Project Number: 2034-22000-014-026-T
Project Type: Trust Fund Cooperative Agreement

Start Date: Jul 1, 2025
End Date: Jun 30, 2026

Objective:
1. Screen locally isolated bacterial strains for biological control efficacy against powdery mildew in grapevines. 2. Perform genome sequencing on effective biological control strains for full identification.

Approach:
Objective 1) Grape plants will be sprayed with bacterial suspensions of locally isolated bacteria that have been identified for ability to produce chitinase enzymes. Plants will be exposed to powdery mildew-infected source plants and evaluated for development of disease symptoms. Bacteria that inhibit development of powdery mildew will be selected for further characterization in Objective 2. Objective 2) Bacterial strains that inhibit development of powdery mildew on grape plants will be identified to the species and strain level by genome sequencing to facilitate further development as biological control products. Sequencing will be conducted by a commercial provider, and genome sequences will be compared to existing bacterial genome collections to identify species.