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ARS Home » Pacific West Area » Parlier, California » San Joaquin Valley Agricultural Sciences Center » Crop Diseases, Pests and Genetics Research » Research » Research Project #442782

Research Project: Identification of Novel Central Valley Trichoderma Isolates for Biological Control of Pierce’s Disease in California

Location: Crop Diseases, Pests and Genetics Research

Project Number: 2034-22000-014-016-R
Project Type: Reimbursable Cooperative Agreement

Start Date: Jul 1, 2022
End Date: Jun 30, 2025

Identify and sequence Central Valley grapevine Trichoderma isolates that exhibit effective biocontrol of Pierce's disease of grapevine.

An already available unique collection of Central Valley Trichoderma isolates will be screened to identify those that enhance resistance to Pierce's disease. This will consist of greenhouse plant-pathogen assays whereby plants are first inoculated with Trichoderma isolates via stem or root inoculations, and then infected with Xylella fastidiosa, the causal agent of Pierce's disease, via the pin-prick method. Following two-, four- and six-months, disease measurements will be made and Xylella fastidiosa titers assessed via PCR in infected plants, and comparisons will be made with healthy controls. Next-generation sequencing and microscopy approaches also will be employed to genetically and morphologically characterize of Trichoderma biocontrol isolates.