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ARS Home » Research » Research Project #449657

Research Project: Expression and Purification of Proteins of Interest for Risk Group 4 Pathogen Reagent Development

Location: Biologics Development Module

Project Number: 3022-32000-026-024-S
Project Type: Non-Assistance Cooperative Agreement

Start Date: Jun 15, 2026
End Date: Jun 14, 2027

Objective:
The objective is to produce purified recombinant proteins of interest for usage in the generation of novel assays and reagent development for Risk Group 4 Pathogens to enhance and develop diagnostic capacities.

Approach:
The USDA will identify proteins of need for the development of novel assays and reagents for development of reagents to Risk Group 4 pathogens and coordinate with the cooperator the selection of five proteins for expression and purification. The cooperator will order the genes for the proteins of interest synthesized and utilize plasmids to express and purify approximately 3mg of purified protein utilizing affinity chromatography.