Project Number: 2072-22000-046-042-S
Project Type: Non-Assistance Cooperative Agreement
Start Date: Sep 1, 2023
End Date: Oct 31, 2024
1. To purify, identify, and optimize extraction procedures for potential nematocidal compounds from S. sisymbriifolium.
Our approach will be to fractionate S. sisymbriifolium plant tissue extracts and test the toxicity of fractions to nematodes by using standard hatching assays. To achieve this goal, a series of extracts will be prepared from flowers, leaves, and roots of freeze-dried plant material at the semi-preparative scale (Gurbuz et al., 2015). After the screening of the extracts for nematode toxicity, the most potent extracts will be fractionated using analytical preparative techniques such as liquid extraction, column separation, and solid-phase extraction. The most potent fractions will be analyzed by high resolution mass spectrometry for specific chemical groups identification. Upon toxicity confirmation, bioactive molecules will be isolated and identified; and extraction procedures for these potential nematicidal compound(s) will be developed thus allowing us to obtain enough material for hatching assays and other bioassays.